Abstract: Fish are the top community in river ecosystems, and spawning and reproduction are the most important stages in the life cycle of fish, which have a significant impact on the stability of aquatic ecosystems. In order to maintain the ecological hydrological processes required for fish reproduction, hydropower stations do not implement peak shaving operation during the spawning and reproduction period of fish, and discharge according to the upstream inflow. The operation mode of "natural rhythm ecological scheduling" has gradually become the norm in the upper reaches of the Yellow River. Based on this requirement, the peak shaving capacity of hydropower stations will be greatly limited during the fish reproduction season. In order to meet the demand for peak shaving capacity of the power grid under the integrated operation of water, wind, and solar power, the storage capacity of conventional hydropower stations is used as the lower reservoir of pumped storage power stations, and a new reservoir is built upstream as the upper reservoir of pumped storage power stations. The construction of an "integrated pumped storage power station" can effectively balance the randomness and volatility of wind and photovoltaic output. This article takes the "Water, Wind, and Solar Energy Storage" integrated renewable energy base in the upper reaches of the Yellow River as the research object, and constructs a multi-source system joint ecological operation model that couples conventional hydropower stations, integrated pumped storage power stations, wind power, and photovoltaic power stations. It quantitatively evaluates the ecological impact of integrated pumped storage power station operation on water level fluctuations and system peak shaving capacity in the reservoir area under typical wind and solar power output scenarios when conventional hydropower stations operate according to runoff during fish reproduction season. The research results quantitatively reveal the trade-off relationship between pumped storage capacity, new energy output characteristics, and water level disturbances in conventional hydropower stations, which can provide technical support for the ecological peak shaving operation of hydropower stations for fish reproduction requirements.